Neoplasms

Symptoms and General Pathology

All Conditions

Skin and Connective Tissue Diseases

Neoplasm Metastasis

Breast Neoplasms

Neoplastic Processes

Breast Diseases

Skin Diseases

Zheng Wang, PhD

Jun Ren, MD

The investigators want to develop a gene expression profile the for prediction the chemotherapeutic response of patients with metastatic breast cancer.

1. Metastatic breast cancer tissue is obtained from core needle biopsies pretreatment and is flash frozen and stored at -70℃ until processing.
2. After patients received docetaxel combination thiotepa for two cycles, response is assessed using Response Evaluation Criteria in Solid Tumor Group (RECIST) guidelines. Degree of response is used to divide the cancers into two groups-sensitive and resistant tumours.
3. The gene expression in metastatic breast cancer tissue sample is detected by microarray to screen gene markers that are differently expressed between groups
4. Statistical analysis is performed using unsupervised hierarchical cluster.

the tissue will be obtained by core niddle biopsy.

Gene Expression Profiling of Metastatic Breast Cancer Predict the Therapeutic Response to Chemotherapy

Response to chemotherapy is assessed using Response Evaluation Criteria in Solid Tumor Group (RECIST) guidelines

Time to disease progression is measured from the date therapy is initiated to the date of documented disease progression.

Overall survival is measured from the date therapy is initiated to the date of death or final follow-up.

clinical benefit response include CR,PR,SD

Peking University Cancer Hospital & Institute

Director

Respiratory Tract (Lung and Bronchial) Diseases

Pleural Effusion, Malignant

Pleural Effusion

Pleural Diseases

Respiratory Tract Diseases

Pleural Neoplasms

Respiratory Tract Neoplasms

Thoracic Neoplasms

All Drugs and Chemicals

Antineoplastic Agents

Analgesics

Immunomodulating Agents

Interleukin-2

Analgesics, Non-Narcotic

The patients are randomized to receive the cytokine infusion in the pleural cavity

cytokine

JUN REN, MD

The investigators want to develop a gene expression profile for the prediction of immunotherapy response of patients with metastatic breast cancer presenting malignant pleural effusion.

1. The patiets with malignant pleural effusion are randomizned to be treated with cytokins(inteleukin 2) or dendritic cells(DC) plus cytokine induced killer cells(CIK) locally.
2. Malignant pleural effusion from metastatic breast cancer patient is obtained through thoracentesis and is centrifugalized to enrich cancer cells before the therapy.
3. The enriched cancer cells are flash frozen and stored at -80℃ until processing.
4. The gene expression in pleural effusion is detected by microarray to screen gene markers that are differently expressed between groups .
5. Statistical analysis is performed using unsupervised hierarchical cluster.

The cancer cells are taken from the malignant pleural effusion of breast cancer patiets.

Gene Expression Profiling of Breast Cancer Cells Predict the Response of Malignant Pleural Effusion

Gene Expression Profiling of Breast Cancer Cells in Pleural Effusion Prodict the Response of Malignant Pleural Effusion to Immunotherapy

Response of malignant pleural effusion to immunotherapy is evaluated with WHO guidelines

compare the immunological status of pleural effusion before and after the therapy

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
Gene Expression Profiling of Metastatic Breast Cancer Predict the Therapeutic Response to Chemotherapy	NCT01198301	Breast Neoplasm... Neoplasm Metast...		18 Years - 75 Years	Peking University Cancer Hospital & Institute
Gene Expression Profiling of Breast Cancer Cells Predict the Response of Malignant Pleural Effusion	NCT01256801	Breast Neoplasm... Neoplasm Metast... Gene Expression... Immunotherapy	cytokine	18 Years - 75 Years	Peking University Cancer Hospital & Institute
Gene Expression Profiling of Breast Cancer Cells Predict the Response of Malignant Pleural Effusion	NCT01256801	Breast Neoplasm... Neoplasm Metast... Gene Expression... Immunotherapy	cytokine	18 Years - 75 Years	Peking University Cancer Hospital & Institute
Gene Expression Profiling of Metastatic Breast Cancer Predict the Therapeutic Response to Chemotherapy	NCT01198301	Breast Neoplasm... Neoplasm Metast...		18 Years - 75 Years	Peking University Cancer Hospital & Institute
Gene Expression Profiling of Metastatic Breast Cancer Predict the Therapeutic Response to Chemotherapy	NCT01198301	Breast Neoplasm... Neoplasm Metast...		18 Years - 75 Years	Peking University Cancer Hospital & Institute

Spots Global Cancer Trial Database for metastatic breast neoplasms