Neoplasms

All Conditions

Respiratory Tract (Lung and Bronchial) Diseases

Rare Diseases

Mesothelioma

Mesothelioma, Malignant

Carcinoma

Neoplasms, Glandular and Epithelial

Neoplasms by Histologic Type

Malignant Mesothelioma

Probe based confocal laser endomicroscopy (Mauna kea technologies) will be used, after intravenous fluorescein injection, for every patients admitted for medical thoracoscopy, to study the pleural cavity. Images will be compared with biopsies

Probe based confocal laser endomicroscope can be introduced through the working chanel of the thoracoscope. this allows the study of the pleural cavity with this new tool.

Study of the pleural cavity with a confocal laser endomicroscope.

Olivier Bonhomme, MD

Probe based confocal laser endomicroscopy (pCLE) is a new optical endoscopic technique, generating fluorescent light emission from the tissue of interest and allowing in vivo live imaging at a cellular level ("optical biopsies"). It was first used in gastroenterology and came later to the light in pulmonary medicine and is still an experimental technique. In gastroenterology, this new investigational technique is used in Barret oesophagus, inflammatory bowel disease, pancreas cystic lesions... Nowadays, there are no data concerning usefulness of endomicroscopy in medical thoracoscopy. During thoracoscopy This new tool could help to target biopsies or help clinicians to do the right diagnosis early, allowing rapid therapeutic intervention (talc pleurodesis for example) . Furthermore, some details can be studied only during live imaging as microorganisms or bloodflows. The investigators performed an endomicroscopy to every patient needing a thoracoscopy (no matter the indication) and who agreed to participate. The pCLE features between malignant and benign pleura were compared in order to find specific criteria for malignant infiltration.

Probe Based Confocal Laser Endomicroscopy During Thoracoscopy for Pleural Malignancies Diagnosis.

Eleven preselected criteria were assessed in their ability to distinguish benign from malignant pleura Qualitative variables are presented in this table

Eleven preselected pCLE criteria were assessed in their ability to distinguish benign from malignant pleura.

Here are presented Mean cell size and maximum vascular diameter

Eleven preselected pCLE criteria were assessed in their ability to distinguish benign from malignant pleura.

Quantitative criteria are presented in this table. Here is presented the mean cellular density

Eleven preselected pCLE criteria were assessed in their ability to distinguish benign from malignant pleura.

Quantitative criteria are presented in this table. Here is presented the vascular density.

The investigators performing the thoracoscopy had to score the pCLE acquisition. Three level of quality were used: Good, Acceptable, Low.

University of Liege

Medical doctor - clinical assistant

Probe based confocal laser endomicroscopy (Mauna kea technologies) will be used, after intravenous fluorescein injection, for every patients admitted for medical thoracoscopy, to study the pleural cavity. Images will be compared with biopsies

Study of the pleural cavity with a confocal laser endomicroscope.: Probe based confocal laser endomicroscope can be introduced through the working chanel of the thoracoscope. this allows the study of the pleural cavity with this new tool.

Patient Aged More Than 18 Years Admitted for Thoracoscopy

Age, Continuous

Sex: Female, Male

Race and Ethnicity were not collected from any participant.

Race and Ethnicity Not Collected

Thoracoscopy indication

Final histological diagnosis

Dr Olivier Bonhomme

The objective of the test is to assess if the pCLE feature "full chia seed sign" is found statistically more frequently in the "benign pleura group" than in the "malignant pleural infiltrations group".

The objective is to assess if the abnormal tissular architecture is significantly more frequently found in the "malignant pleural infiltrations group" than in the "benign pleura group".

The objective is to assess if the pCLE feature "cellular shape homogeneity" is found statistically more frequently in the "benign pleura group" than in the "malignant pleural infiltrations group".

The objective is to assess if dysplastic vessels are more frequently found in the "malignant pleural infiltrations group" than in the "benign pleura group".

Abnormal tissular architecture

Cellular size homogeneity

Cellular shape homogeneity

Cellular fluorescence homogeneity

Blood vessels dysplasia

Organized connective tissue

Chia seed sign

Participants with a benign pleura histological diagnosis.

Benign Pleura

Participants with a malignant pleural disease demonstrated by biopsies.

Malignant Pleural Infiltrations

Pleural Carcinomatosis Identification (Compared to Standard Biopsies). Qualitative Criteria.

Mean cell size

Maximal vascular diameter

Some criteria were not assessable for some patients.

Spots Global Cancer Trial Database for optical biopsies