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Cigarette consumption in the context of their habit (not related to the particiapion under study).

Electronic cigarette consumption in the context of their habit (not related to the particiapion under study).

Nicotine replacement therapy (only patch) consumption in the context of their habit (not related to the particiapion under study).

No consumption in the context of their habit (not related to the particiapion under study).

Collection of 5 mL of urinary sample.The pregnancy test is done in each investigator center.

pregnancy test

Collection of 10 mL of urinary sample for cotinine analysis. The analysis is maid during the study and is centralised at the hospital Paul Brousse.

urinary cotinine assesment

Collection of 20 mL of urinary sample for NNN analysis. The analysis is maid at the end of the study and is centralised in a laboratory located at Strasbourg.

Urinary NNN assesement

This questionnaire will permit to assess the smoking habits of the participants.

Questionnaire (Fagerstrom test)

This test will permit to determinate if the participant are axposed to passive smoking or not.

Measurement of carbon monoxide levels expired

Patrick DUPONT, MD

Patrick Dupont, Dr

Patrick Dupont, MD

Multicenter, national study, non-randomized , controlled, comparative with 4 independent groups (20 participants in each group). There are 3 experimental groups ("smocker", "nicotine replacement therapy" an "electronic cigarette") and 1 control group ("without nicotine").

The main objective of the study is to compare the level of urinary NNN in the electronic cigarette users versus the nicotine replacement therapy (patch) users.

The study has only one visit taking place as below:

* Verification of inclusion and non-inclusion criteria.
* Collection of the informed consent of the subject Given the non-invasive nature of the procedure, a period of reflection will be given to the subjects, however, information and obtaining consent will be made during the same visit.
* Collection of 5 mL of required for the pregnancy test in women of childbearing age. This collection does not need to be fasting. If this test is positive, the subject will not be included in the study.
* Collection of 30 mL of urine needed for the cotinine and the NNN quantitative analysis.

This collection does not need to be fasting.

Then , the urine sample is divided into two vials stored at -80 ° C:

* a 10 mL vial for the cotinine analysis,
* a 20 mL vial for the NNN analysis.

  * Realization of the Fagerstrom test (FTCD) in order to evaluate subject smoking. This test is not done for the group 4 (group "without nicotine")
  * Measurement of carbon monoxide in exhaled air If after signing the consent, the subject realizes that he cannot urinate or he did not enough urine during the inclusion visiting, the subject can come back the next working day to make the urine collection.

Determination of Carcinogens Nicotine in Electronic Cigarettes Users

The determination of urinary NNN of will be performed at the end of the study only for subjects with cotinine / creatinine ratio adequate.

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
Determination of Carcinogens Nicotine in Electronic Cigarettes Users	NCT02897401	Smoking Carcinogenesis ...	pregnancy test urinary cotinin... Urinary NNN ass... Questionnaire (... Measurement of ...	18 Years -	Assistance Publique - Hôpitaux de Paris
Determination of Carcinogens Nicotine in Electronic Cigarettes Users	NCT02897401	Smoking Carcinogenesis ...	pregnancy test urinary cotinin... Urinary NNN ass... Questionnaire (... Measurement of ...	18 Years -	Assistance Publique - Hôpitaux de Paris
Determination of Carcinogens Nicotine in Electronic Cigarettes Users	NCT02897401	Smoking Carcinogenesis ...	pregnancy test urinary cotinin... Urinary NNN ass... Questionnaire (... Measurement of ...	18 Years -	Assistance Publique - Hôpitaux de Paris

Spots Global Cancer Trial Database for carcinogenesis of the nicotine