Neoplasms

Symptoms and General Pathology

All Conditions

Neoplastic Cells, Circulating

Neoplasm Metastasis

Neoplastic Processes

Tai-Horng Young

Yin-Tzu Chen

This culture system utilizes the special affinity difference of biomedical material coating for different cells to achieve the effect of isolating tumor cells from the blood sample. The coating of the system has the characteristic that to make the WBCs adhesion, but the cancer cells in the blood sample suspend in the culture medium, which achieves the effect of separating cancer cells from the blood. The supernatant with the cancer cells can further be isolated from the cultural system for related analysis and detection to achieve early diagnosis and screening.

Culture System for Isolating Circulating Tumor cells_2

Culture System for Isolating Circulating Tumor Cells

The number of circulating cancer cells from the blood sample will be investigated. To distinguish cancer cells from blood cells for the system, the lung cancer cells were immunofluorescence stained for Cytokeratin, white blood cells were stained for CD45 and nuclei were counterstained with DAPI.

The cell isolation efficiency was characterized by spiking A549 lung cancer cells with celltracker at the concentrations 1000, 100 and 10 cells into whole blood.

National Taiwan University Hospital

Digestive System Diseases

Recurrence

Neoplasm, Residual

Colonic Neoplasms

Colorectal Neoplasms

Intestinal Neoplasms

Gastrointestinal Neoplasms

Digestive System Neoplasms

Gastrointestinal Diseases

Colonic Diseases

Intestinal Diseases

Adjuvants, Anesthesia

Analgesics

Central Nervous System Depressants

All Drugs and Chemicals

Herbal and Botanical

Sufentanil

Bupivacaine

Morphine

Dsuvia

Pirinitramide

Analgesics, Opioid

Narcotics

Cola

Patients will receive perioperative epidural analgesia. Drugs: bupivacaine 1.25 mg/ml and sufentanil 0.5 mcg/ml Form and frequency: continuous infusion Dosage: 4 - 14 ml/h with boluses 2 - 4 ml based on pain assessment Duration: as long as required

Patients will receive postoperative analgesia with piritramide. Drugs: piritramide 1.0 mg/ml Form and frequency: continuous infusion Dosage: 0 - 4 ml/h with boluses 2 - 4 ml based on pain assessment Duration: as long as required

Patients will receive postoperative analgesia with morphine. Drugs: morphine 1.0 mg/ml Form and frequency: continuous infusion Dosage: 0 - 4 ml/h with boluses 2 - 4 ml based on pain assessment Duration: as long as required

Epidural analgesia

Piritramide

Emil Berta, MD PhD

The aim of this study is to compare the effects of three types of perioperative analgesia on the number of circulating cancer cells (representing minimal residual disease) following radical colon cancer surgery. Patients will be randomized into one of three groups. The intervention group will receive combined regional and general anesthesia during surgery and postoperative epidural analgesia. The two control groups will receive balanced general anesthesia and either morphine-based or piritramide-based postoperative analgesia. We hypothesize that epidural analgesia will be favorable to both piritramide-based and morphine-based analgesia and that piritramide-based analgesia will be favorable to morphine-based analgesia with regard to the number of circulating cancer cells and its development in the early postoperative period.

Techniques of regional analgesia such as epidural analgesia may favorably influence metastasis development following cancer surgery compared to analgesia based on strong opioids such as morphine or piritramide. These beneficial effects, if present, are probably attributable to less immunosuppression of antimetastatic immune defenses.

The aim of this study is to identify techniques of perioperative analgesia with the potential to prevent metastasis development in patients undergoing open radical colon cancer surgery. In the early postoperative period, a relationship between metastasis development and the number of circulating cancer cells representing minimal residual disease has been shown. Therefore, effects of epidural, morphine-based and piritramide-based analgesia on the number of circulating cancer cells will be compared at several time points during the peroperative and early postoperative periods. The number of circulating cancer cells will be assessed in peripheral venous blood samples using real-time polymerase chain reaction. Perioperative care will be standardized and patients will be followed by clinical observation, laboratory analyses and monitoring instrumentation daily during their hospital stay.

Effects of Different Types of Perioperative Analgesia on Minimal Residual Disease Development After Colon Cancer Surgery

Number of circulating cancer cells will be measured in venous blood samples. The quantitative real-time polymerase chain reaction using carcinoembryonic antigen and cytokeratine 20 as markers for circulating cancer cells will be used for minimal residual disease detection.

Self reported pain intensity. Scale: 0 = no pain, 10 = worst pain imaginable. A score 0-10 will be recorded every four hours.

Brno University Hospital

Tomas Bata Hospital, Czech Republic

The Institute of Molecular and Translational Medicine, Czech Republic

Senior Researcher

Urinary Tract, Sexual Organs, and Pregnancy Conditions

Urinary Bladder Neoplasms

Urologic Neoplasms

Urogenital Neoplasms

Urogenital Diseases

Female Urogenital Diseases

Pregnancy Complications

Female Urogenital Diseases and Pregnancy Complications

Urinary Bladder Diseases

Urologic Diseases

Male Urogenital Diseases

Arm of patients undergoing radical cystectomy for bladder cancer providing peripheral blood samples for detection of circulating cancer cells

Arm of healthy subjects not undergoing radical cystectomy providing peripheral blood samples for detection of circulating cancer cells

Ficoll based isolation of mononuclear cells from the peripheral blood of patients undergoing radical cystectomy, nucleic acids extraction and, finally, PCR based detection of sequences specific to cytokeratins.

Detection of circulating tumor cells in blood samples

Vincenzo VP Pagliarulo, Urologist

Very few factors may be identified as prognostic for patients with bladder cancer undergoing radical cystectomy. Recently, detection of circulating tumor cells has shown to be very promising in anticipating both the likelyhood of distant metastases and survival in patients with breast cancer, melanoma, prostate cancer and other malignancies. In the present study we both tested the detection rate of circulating tumor cells using a PCR based methodology in the peripheral blood of patients undergoing radical cystectomy, and we further correlated our results with their clinical outcome.

Detection and Clinical Significance of Circulating Cancer Cells in Patient Undergoing Radical Cystectomy

This outcome measure reflects the exact number of subjects diagnosed with circulating tumor cells within the total population of subjects included in the study

Comparison of overall survival between the population of patients found with circulating tumor cells and those found without circulating tumor cells

Azienda Ospedaliero-Universitaria Consorziale Policlinico di Bari

Medical Doctor

Prostatic Neoplasms

Subjects receiving cryotherapy for prostate cancer

Subjects receiving radiation for prostate cancer

Prostate cryotherapy

Radical prostatectomy

Radiation

Yeong-Shiau Pu, PhD

Yeong-Shiau Pu, Prof.

Chung-Hsin Chen, MD

HJ Yu, Prof.

CY Huang, MD

SM Wang, MD

HC Tai, MD

SP Lui, MD

WJ Lin, MD

JS Chen, MD

The change of immune profiles and existence of circulating tumor cells following prostate cryotherapy may be correlated with the clinical outcome.

Immune Profiles and Circulating Tumor Cell Status Following Prostate Cryotherapy

Includes:

1. Biochemical (PSA) recurrence according to the ASTRO or Phoenix criteria for patients undergoing cryotherapy (Cryo) or radiotherapy (RT)
2. Prostate biopsy positivity
3. Radiographic evidence of recurrence (CT/MRI, Bone scan, radiographs, etc.) Time to recurrence defined by the either of the above 3 outcome parameters

according to the QLQ C30 and PR25 questionnaire interview

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
Culture System for Isolating Circulating Tumor cells_2	NCT04125667	Early Diagnosis Diagnostic Tech...		20 Years - 70 Years	National Taiwan University Hospital
Effects of Different Types of Perioperative Analgesia on Minimal Residual Disease Development After Colon Cancer Surgery	NCT02314871	Colon Cancer Minimal Residua...	Epidural analge... Piritramide Morphine	18 Years -	The Institute of Molecular and Translational Medicine, Czech Republic
Culture System for Isolating Circulating Tumor cells_2	NCT04125667	Early Diagnosis Diagnostic Tech...		20 Years - 70 Years	National Taiwan University Hospital
Detection and Clinical Significance of Circulating Cancer Cells in Patient Undergoing Radical Cystectomy	NCT02345473	Bladder Cancer Circulating Tum...	Detection of ci...	18 Years -	Azienda Ospedaliero-Universitaria Consorziale Policlinico di Bari
Effects of Different Types of Perioperative Analgesia on Minimal Residual Disease Development After Colon Cancer Surgery	NCT02314871	Colon Cancer Minimal Residua...	Epidural analge... Piritramide Morphine	18 Years -	The Institute of Molecular and Translational Medicine, Czech Republic
Culture System for Isolating Circulating Tumor cells_2	NCT04125667	Early Diagnosis Diagnostic Tech...		20 Years - 70 Years	National Taiwan University Hospital
Immune Profiles and Circulating Tumor Cell Status Following Prostate Cryotherapy	NCT01454037	Prostate Cancer Immune Profile Circulating Can... Clinical Corela...	Prostate cryoth... Radical prostat... Radiation	20 Years -	National Taiwan University Hospital

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