Neoplasms

Skin and Connective Tissue Diseases

All Conditions

Symptoms and General Pathology

Rare Diseases

Melanoma

Neoplastic Cells, Circulating

Neuroendocrine Tumors

Neuroectodermal Tumors

Neuroectodermal Tumors, Primitive

Neoplasms, Germ Cell and Embryonal

Neoplasms by Histologic Type

Neoplasms, Nerve Tissue

Nevus, Pigmented

Nevus

Nevi and Melanomas

Skin Neoplasms

Skin Diseases

Neoplasm Metastasis

Neoplastic Processes

Neuroendocrine Tumor

Neuroepithelioma

Dermatologic Agents

All Drugs and Chemicals

Patients that have been hospitalized at the Nîmes University Hospital and who do not have dermatological cancer.

This cohort includes patients with metastatic melanoma.

Laurent Meunier, MD

The main objective of this study is to compare results for the detection of circulating melanoma cells (CMC) using CellSearch versus EPISPOT (EPithelial ImmunoSPOT) techniques between a group of patients with metastatic melanoma and a group of hospitalized control patients.

The secondary objectives of this study include:

A. To compare the following elements between the two patient groups:

* the number of CMCs per ml of blood as determined by EPISPOT
* the number of CMCs per ml of blood as determined by CellSearch
* the percentage of patients with at least 2 CMCs per ml of blood according to the two techniques
* the % of CMCs expressing KI67
* the % of CMCs expressions S100 (only the EPISPOT technique)

B. To compare the EPISPOT and CellSearch techniques is terms of the following:

* the number of CMCs detected per ml blood
* the number of CMCs expressing antigen KI67

C. To re-evaluate the 2-CMC per ml blood threshold

Circulating Tumor Cells and Melanoma: Comparing the EPISPOT and CellSearch Techniques

Circulating Tumor Cells and Melanoma: Comparing the EPISPOT (EPithelial ImmunoSPOT) and CellSearch Techniques

Presence/absence of at least 2 CMCs per ml blood, using both the Epispot and the CellSearch techniques

The number of CMCs per ml blood as determined by the Epispot technique

The number of CMCs per ml blood as determined by the CellSearch technique

The difference between the number of CMCs per ml blood detected with the CellSearch technique and the Epispot Technique (CellSearch - Epispot)

The % difference between the number of CMCs per ml blood detected with the CellSearch technique and the Epispot Technique (CellSearch - Epispot)/CellSearch\*100

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
Circulating Tumor Cells and Melanoma: Comparing the EPISPOT and CellSearch Techniques	NCT01558349	Metastatic Mela...		18 Years -	Centre Hospitalier Universitaire de Nīmes
Circulating Tumor Cells and Melanoma: Comparing the EPISPOT and CellSearch Techniques	NCT01558349	Metastatic Mela...		18 Years -	Centre Hospitalier Universitaire de Nīmes

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