Neoplasms

Symptoms and General Pathology

All Conditions

Respiratory Tract (Lung and Bronchial) Diseases

Neoplasm Metastasis

Pleural Effusion, Malignant

Pleural Effusion

Carcinoma

Pleural Diseases

Respiratory Tract Diseases

Neoplasms, Glandular and Epithelial

Neoplasms by Histologic Type

Pleural Neoplasms

Respiratory Tract Neoplasms

Thoracic Neoplasms

Central Nervous System Depressants

All Drugs and Chemicals

Anesthetics

Patients with malignant pleural effusion who underwent pleural biopsies and blood tests under general anesthesia

Interventions will consist of a diagnostic and symptomatic videothoracoscopy in patients with pleural metastasis under general anesthesia, as part of a standard of care procedure. Fragments of these pleural biopsies (one for routine diagnosis and two for scientific purposes) will enrich the biocollection. Five blood samples will be taken at the time of surgery (3 to 5 ml / tube). These biological samples will be stored in the HCL CRB tumor bank. At the same time, patient demographic data will be collected and anonymized. In the long term, the biological material will be used by IARC for carrying out multi-omics and bioinformatics analyzes

Conserving routinely performed pleural biopsies in our biological resources center to realize multiomics and bioinformatics analyzes secondarily

Gabrielle Drevet, MD

Jean-Michel Maury, MD

Malignant pleural effusion is a common evolution of various cancers and is associated with poor prognosis and quality of life. About 28% of patients with primary malignancy will develop pleural metastasis. Malignant pleural effusion mostly occurs in lung, breast, ovarian and gastric cancers. Median survival ranges from 3 to 13 months according to primary malignancy. Currently, the therapeutic approach is mainly palliative with videothoracoscopic talc pleurodesis or indwelling pleural catheters insertion eventually associated with systemic chemotherapy if patient's general condition allows.

In a early-disseminated tumor cells profile, metastatic cells can accumulate alterations at a distant site and have a different profil from the original tumor cells. Metastatic cells can also accumulate alterations in the course if systemic treatments. Consequently, they may respond differently to drugs.

Recently, EGFR mutations and ALK status discordance between primary tumors and pleural metastases have been demonstrated in a significant portion of lung adenocarcinomas. These studies, realized on malignant pleural effusion isolated cells, enabled us to hypothesize a possible intratumoral heterogeneity within pleural metastases, but no study has been carried out on pleural tissue.

Our aim is to create a biocollection with tissues from pleural carcinomatosis in order to subsequently allow multiomics and bioinformatics analyzes and to characterize a possible intratumoral heterogeneity in pleural metastasis.

Between 3 and 5 pleural biopsies and 5 blood samples for each patient

Pleural Carcinomatosis Tissue Banking

Creation of a Pleural Carcinomatosis Tissue Bank

Blood samples are collected and a piece of biopsy will be used for the constitution of a specific collection in the CRB (Centre de ressource biologique)

Hospices Civils de Lyon

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
Pleural Carcinomatosis Tissue Banking	NCT04844827	Pleural Effusio...	Conserving rout...	18 Years - 110 Years	Hospices Civils de Lyon
Pleural Carcinomatosis Tissue Banking	NCT04844827	Pleural Effusio...	Conserving rout...	18 Years - 110 Years	Hospices Civils de Lyon

Spots Global Cancer Trial Database for conserving routinely performed pleural biopsies in our biological resources center to realize multiomics and bioinformatics analyzes secondarily