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Angiogenin

20 healthy age- and sex- matched controls

Blood samples were obtained at time of diagnosis and will be analyzed for the levels of biomarker candidate molecules.

Obtaining blood samples

Gurcan Gunaydin, MD, PhD

Nilda Suslu

The aim of this study was to determine serum levels of VEGF, sVEGFR1, VEGFR2, IGFBP-3, angiogenin and endoglin in patients with larynx squamous cell carcinoma; investigate their association with clinical parameters and determine their diagnostic and prognostic value.

Although there has been a significant increase in survival in many other cancer types through the years, no significant increase has been achieved in laryngeal SCC survival rates in the last 50 years. Despite the advancements in surgical techniques, organ preservation protocols and multidisciplinary approach, significant amount of patients have been living with morbidity or dying due to recurrence and metastasis. This lack of significant improvement in mortality rates creates the need for reliable and accurate biomarkers in early diagnosis, treatment and follow-up. Considering that the prognosis of two patients at the same clinical stage and treated with the same treatment protocol may differ, it suggests that there may be some differences at the molecular level apart from clinical stages.

This study was mainly prepared to determine serum levels of VEGF, sVEGFR1, VEGFR2, IGFBP-3, angiogenin and endoglin in patients with larynx squamous cell carcinoma; investigate their association with clinical parameters and determine their diagnostic and prognostic value.

To reach these aims, 60 patients who were prospectively and consecutively recruited from those who admitted to Hacettepe University, Department of Otorhinolaryngology, Ankara, Turkey, and were diagnosed with LSCC between May 2018 and February 2020. 20 healthy and age-matched controls were chosen from the hospital staff and relatives of the patients. Serum samples were obtained from all participants at the time of diagnosis, centrifuged and stored at -80 C. ELISA method will be used to analyze the serum levels of VEGF, sVEGFR1, VEGFR2, IGFBP-3, angiogenin and endoglin.

Data analysis will reveal if there are any association between biomarker candidate molecules and clinical parameters.

An Investigation of Biomarker Candidate Molecules in Laryngeal Carcinoma

An Investigation of Clinical Parameters and Biomarkers Associated With Angiogenesis, Proliferation, Cell Adhesion and Invasion in Laryngeal Carcinoma

Serum levels of:

VEGF in pg/ml

sVEGFR1 in pg/ml

VEGFR2 in pg/ml

IGFBP-3 in pg/ml

angiogenin in pg/ml

and endoglin in pg/ml

will be determined in all participants

Disease stage as stage as early (I-II) or late (III-IV)

Receiver operating characteristic analysis will be performed to determine a significant level of any biomarker candidate molecules for diagnostic sensitivity and sensitivity.

Sensitivity and specifity as percentages (%)

Tumor localization as glottic/supraglottic or transglottic

Treatment modality as surgical, non-surgical or combined

All the patients were contacted for survival analysis.

Overall and disease-free survival will be measured in months and survival rates will be calculated in percentages (%).

Overall survival and disease-free survival rates will be calculated according to levels of biomarker candidate molecules and assessed for any association with any molecule.

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
An Investigation of Biomarker Candidate Molecules in Laryngeal Carcinoma	NCT05217147	Larynx Cancer Larynx Carcinom...	Obtaining blood...	-	Hacettepe University
An Investigation of Biomarker Candidate Molecules in Laryngeal Carcinoma	NCT05217147	Larynx Cancer Larynx Carcinom...	Obtaining blood...	-	Hacettepe University

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