Neoplasms

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Salivary Gland Neoplasms

Adenoma, Pleomorphic

Neoplasms, Glandular and Epithelial

Neoplasms by Histologic Type

Mouth Neoplasms

Head and Neck Neoplasms

Mouth Diseases

Stomatognathic Diseases

Salivary Gland Diseases

Oral Cancer

carcinoma ex pleomorphic adenoma of major and minor salivary glands.

Benign pleomorphic adenoma of major and minor salivary glands.

normal controls obtained from border of excision biopsy of mucocele in the lip mucosa of healthy individuals.

new strategy where gold nanoparticles synthetized and added to CD24 primer forming CD24-Gold Nanocomposite to be used for detection of cancer stem cells

CD24-Gold Nanocomposite expression using Real-time quantitative polymerase chain reaction

conventional strategy using non-conjugated CD24 expression to used for detection of cancer stem cells

non-conjugated CD24 expression using Real-time quantitative polymerase chain reaction

Amina F Farag, PhD

Nano-based diagnostic tool can provide promising highly sensitive, specific biomarker for early detection and treatment of salivary gland tumours compared to non-conjugated biomarkers and in turn improves patient prognosis and outcome.

Cancer stem cells form a small subset of highly tumorigenic cells within the bulk of the tumours which mainly responsible for initiation, invasion, rapid growth, metastasis and therapeutic resistance in different types of human cancers. Nowadays, nanotechnology has increasing attention in multi-disciplinary research fields. Conjugated gold nanoparticles are widely used as biomarkers and bio-delivery vehicles in the medicine as well as early and advanced cancer detection and treatment. The current work aimed to introduce a novel diagnostic and prognostic approach in early detection of cancer stem cells in salivary gland tumours using gold nanoparticles conjugated to CD24 (CD24-Gold Nanocomposite).

Briefly, a portion of surgically resected salivary gland tumour specimens was immersed immediately in liquid nitrogen, allowed to snap frozen and stored at -80 C (Frozen tissue) while the other portion was fixed in 10% buffered formalin solution and embedded in paraffin (Formalin-fixed paraffin-embedded tissue)

Diagnostic and Prognostic Accuracy of Gold Nanoparticles in Salivary Gland Tumours

Gold Nanoparticles as Novel Biomarkers for Cancer Stem Cells in Salivary Gland Tumors: A Diagnostic and Prognostic Accuracy Study

we tested the differential expression of CD24-AuNC and non-conjugated CD24 biomarkers in all studied groups in order to identify the most sensitive and specific diagnostic biomarker to be used in detecting salivary gland tumours,

We investigated the relationship between both biomarkers' expression and clinicopathological characteristics of PA and CXPA patients such as age, gender, tumor site, tumor size (maximum diameter in mm), histopathological subtype, encapsulation, degree of invasion, facial nerve involvement and lymph node (LN) metastasis that may have direct relation with the tumor prognosis

we assessed the disease progression and/or patient survival and examined its association with biomarkers expression

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
Diagnostic and Prognostic Accuracy of Gold Nanoparticles in Salivary Gland Tumours	NCT04907422	Carcinoma Ex Pl... Pleomorphic Ade...	CD24-Gold Nanoc... non-conjugated ...	-	October 6 University
Diagnostic and Prognostic Accuracy of Gold Nanoparticles in Salivary Gland Tumours	NCT04907422	Carcinoma Ex Pl... Pleomorphic Ade...	CD24-Gold Nanoc... non-conjugated ...	-	October 6 University

Spots Global Cancer Trial Database for pleomorphic adenoma of salivary glands