Neoplasms

All Conditions

Digestive System Diseases

Rare Diseases

Carcinoma

Gallbladder Neoplasms

Neoplasms, Glandular and Epithelial

Neoplasms by Histologic Type

Biliary Tract Neoplasms

Gastrointestinal Neoplasms

Digestive System Neoplasms

Biliary Tract Diseases

Gastrointestinal Diseases

Gallbladder Diseases

Gallbladder Cancer

Cases will be the patients with newly-diagnosed gallbladder carcinoma.

The controls will be matched （1：1）by sex，race and age which will be selected from the patients receiving cholecystectomy due to gallstones from the same hospital with the cases.

yingbin liu

This research will be the first study for exosome purified from blood in gallbladder carcinoma patients. Proteomics studies will be done in both tumor tissue and the circulating exosome from blood specimens. Then, the potential prognostic and predictive biomarkers will be searched by bioinformatics to find the correlations between exosome biomarkers and gallbladder carcinoma.

Background:

Exosomes are formed by inward budding of late endosomes, producing multivesicular bodies (MVBs), and are released into the environment by fusion of the MVBs with the plasma membrane. It has been demonstrated that the content and function of exosomes depends on the originating cell and the conditions under which they are produced. Tumor exosome production, transfer and education of bone marrow cells supports tumor growth and metastasis.

Aims of the study:

Proteomics studies will be done in both tumor tissue and the circulating exosome from the gallbladder carcinoma patients. Then, the potential prognostic and predictive biomarkers will be searched by bioinformatics to find the correlations between exosome biomarkers and gallbladder carcinoma.

Materials and Methods:

The investigators will conduct a case-control study in 11 hospitals from China. Cases will be the patients with newly-diagnosed gallbladder carcinoma. The controls will be matched （1：1）by sex，race and age which will be selected from the patients receiving cholecystectomy due to gallstones from the same hospital with the cases. A total of 50 patients with gallbladder carcinoma will be recruited. The investigators will obtain the blood and tumor tissue samples for further analysis of proteomics studies. Exosome from blood specimens will be isolated and purified by sucrose gradient ultracentrifugation. The potential prognostic and predictive biomarkers will be searched by bioinformatics to find the correlations between exosome biomarkers and gallbladder carcinoma. Data will be collected from January 2018 to December 2018.

A Study of Circulating Exosome Proteomics In Gallbladder Carcinoma Patients

Proteomics studies will be done in both tumor tissue and the circulating exosomes from blood specimens. The specific protein A in the circulating exosomes from the gallbladder carcinoma patients will be selected for further analysis.

The expression of protein A （which is selected from outcome 1） in the circulating exosomes from patients will be detected by flow cytometry analysis. The ROC curve of A+ circulating exosomes will be done to compare with the clinical standard tumour biomarker (e.g. CA199) for patients with gallbladder carcinoma patients.

The First Hospital of Jilin University

Shanghai Zhongshan Hosiptal

First Affiliated Hospital Bengbu Medical College

Second Affiliated Hospital of Nanchang University

The Affiliated Hospital of Inner Mongolia Medical University

First Affiliated Hospital of Xinjiang Medical University

The First Affiliated Hospital of Shanxi Medical University

The affiliated hospital of Jining medical college

The Affiliated Hospital of Tianjin Medical University

The first affiliated hospital of Zhongshan university

liu yingbin

vice-president，Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

Symptoms and General Pathology

Postoperative Complications

Anastomotic Leak

Rectal Neoplasms

Colorectal Neoplasms

Intestinal Neoplasms

Intestinal Diseases

Rectal Diseases

Herbal and Botanical

All Drugs and Chemicals

Cola

Preservation of left colonic artery in rectal cancer surgery.

The left colonic artery was dissected in rectal cancer surgery

Preserve the left colonic artery

The left colonic artery was dissected in rectal cancer surgery.

The left colonic artery is not preserved

Wei d tong, PhD

To evaluate the feasibility and clinical significance of preserving left colonic artery in rectal cancer surgery.The investigators will focus on the effect of preserving left colonic artery during radical resection of rectal cancer on anastomotic leakage and oncology efficacy.

The investigators performed three-dimensional (3D) reconstruction to investigate the vascular anatomy, including the inferior mesenteric artery (IMA) and left colic artery (LCA),to help make pre-operative strategies of rectal cancer surgery.The investigators will preserving the left colonic artery during rectal cancer surgery and evaluate its feasibility and clinical significance.The investigators will focus on the effect of preserving left colonic artery during radical resection of rectal cancer on anastomotic leakage and oncology efficacy.

Preserving Left Colonic Artery During Radical Resection of Rectal Cancer

A Clinical Cohort Study on the Effect of Preserving Left Colonic Artery During Radical Resection of Rectal Cancer on Anastomotic Leakage and Oncology Efficacy

The incidence of anastomotic leakage within 30 days after surgery was compared between the intervention group and the control group.

The incidence of distant metastasis rectal cancer within two years after surgery was compared between the intervention group and the control group.

The incidence of local recurrence rectal cancer within two years after surgery was compared between the intervention group and the control group.

The 5-year survival rates of rectal cancer in the intervention group and the control group were compared.

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
A Study of Circulating Exosome Proteomics In Gallbladder Carcinoma Patients	NCT03581435	Proteinosis Gallbladder Car...		18 Years -	Shanghai Jiao Tong University School of Medicine
Preserving Left Colonic Artery During Radical Resection of Rectal Cancer	NCT03776370	Postoperative C... Proteinosis	Preserve the le... The left coloni...	18 Years - 80 Years	Third Military Medical University
A Study of Circulating Exosome Proteomics In Gallbladder Carcinoma Patients	NCT03581435	Proteinosis Gallbladder Car...		18 Years -	Shanghai Jiao Tong University School of Medicine

Spots Global Cancer Trial Database for proteinosis

Every month we try and update this database with for proteinosis cancer trials from around the world to help patients and their families find trials that might be right for them.
We offer this 100% free of charge and do not endorse any of the trials listed here, we hope it helps you or a loved one.

Spots Global Cancer Trial Database for proteinosis

Every month we try and update this database with for proteinosis cancer trials from around the world to help patients and their families find trials that might be right for them. We offer this 100% free of charge and do not endorse any of the trials listed here, we hope it helps you or a loved one.

Every month we try and update this database with for proteinosis cancer trials from around the world to help patients and their families find trials that might be right for them.
We offer this 100% free of charge and do not endorse any of the trials listed here, we hope it helps you or a loved one.