Neoplasms

All Conditions

Digestive System Diseases

Gland and Hormone Related Diseases

Nervous System Diseases

Rare Diseases

Carcinoma

Stomach Neoplasms

Pancreatic Neoplasms

Carcinoma, Ductal

Sarcoma

Brain Neoplasms

Colorectal Neoplasms

Neoplasms, Glandular and Epithelial

Neoplasms by Histologic Type

Intestinal Neoplasms

Gastrointestinal Neoplasms

Digestive System Neoplasms

Gastrointestinal Diseases

Colonic Diseases

Intestinal Diseases

Rectal Diseases

Neoplasms, Connective and Soft Tissue

Stomach Diseases

Central Nervous System Neoplasms

Nervous System Neoplasms

Brain Diseases

Central Nervous System Diseases

Endocrine Gland Neoplasms

Pancreatic Diseases

Endocrine System Diseases

Stomach Cancer

Pancreatic Cancer

Bile Duct Cancer

Soft Tissue Sarcoma

Intrahepatic Cholangiocarcinoma

Determine the feasibility and clinical utility of performing Human Intravital Microscopy (HIVM) in patients with solid tumors during surgical resection.

Undergo Intravital microscopy (IVM), which allows real-time, direct visualization of microscopic blood vessels and calculation of blood flow.

Diagnostic Microscopy

Fluorescein Sodium Injection

Emmanuel M Gabriel, M.D., Ph.D.

This study will investigate the tumor-associated vasculature of patients with solid tumors. The investigators will use a technology known as intravital microscopy (IVM) in order to visualize in real-time the vessels associated with solid tumors. The IVM observations may determine if an individual patient's tumor vessels would be amenable to receiving systemic therapy, based on the functionality of the vessels.

PRIMARY OBJECTIVE: To determine the feasibility and clinical utility of performing HIVM in patients with solid tumors during standard course of treatment (surgical resection).

SECONDARY OBJECTIVES:

1. Compare the microscopic observation of the tumor-associated vessels with normal tissue (e.g. peritoneal surface or normal brain tissue) in each individual subject.
2. Correlate the microscopic observations of the tumor-associated vessels with pathologic grade of tumor.
3. To correlate the microscopic observation of the microvasculature with tumor-specific and overall survival.

OUTLINE:

Patients receive fluorescein intravenously (IV) and undergo HIVM over 1-2 minutes per field.

After completion of study, patients are followed up at 2-3 weeks after surgery.

This is an open-label, non-randomized, single center, study of IVM observation in conjunction with fluorescein in subjects with solid tumors undergoing surgical resection.

Intravital Microscopy in Human Solid Tumors

Intravital Microscopy (IVM) in Human Solid Tumors

Identify and measure vessels associated with solid tumors

Visualize vital dye within the vessels (fluorescein)

Calculate the blood flow velocity of the vessels and tissue penetration of fluorescent dye as a marker of vessel permeability.

Post-operative comparison of the microvasculature of tumor with normal tissue (e.g. peritoneum) in each individual subject using vessel diameters, vessel density, detection of intravital dye and flow rates.

The investigators will determine if there is a correlation between the microvasculature with pathologic features of the tumor implants (i.e. tumor grade) at the time of the final pathology report (5-7 days after surgery).

The investigators will determine if there is a correlation between the microscopic observation of the tumor microvasculature tumor-specific and overall survival.

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
Intravital Microscopy in Human Solid Tumors	NCT03823144	Solid Tumor, Ad... Clinical Stage ... Gastric Carcino... Malignant Solid... Metastatic Colo... Metastatic Gast... Metastatic Prim... Metastatic Sarc... Postneoadjuvant... Resectable Colo... Resectable Live... Resectable Panc... Resectable Sarc... Stage IV Colore...	Diagnostic Micr... Fluorescein Sod...	18 Years -	Mayo Clinic
Intravital Microscopy in Human Solid Tumors	NCT03823144	Solid Tumor, Ad... Clinical Stage ... Gastric Carcino... Malignant Solid... Metastatic Colo... Metastatic Gast... Metastatic Prim... Metastatic Sarc... Postneoadjuvant... Resectable Colo... Resectable Live... Resectable Panc... Resectable Sarc... Stage IV Colore...	Diagnostic Micr... Fluorescein Sod...	18 Years -	Mayo Clinic

Spots Global Cancer Trial Database for resectable liver and intrahepatic bile duct carcinoma