Neoplasms

All Conditions

Rare Diseases

Carcinoma

Carcinoma, Squamous Cell

Neoplasms, Glandular and Epithelial

Neoplasms by Histologic Type

Neoplasms, Squamous Cell

Oral Squamous Cell Carcinoma

Anti-Infective Agents

Central Nervous System Depressants

All Drugs and Chemicals

Ethanol

Healthy subjects having smoked 100 cigarettes or more during their life.

Healthy subjects having smoked less than 100 cigarettes during their life.

Oral epithelial cells will be collected during routine odontology appointments, upon documented non-opposition, via minimally invasive cytobrush sampling of the inner cheek

Oral cytobrush

Brigitte MD GROSGOGEAT

Pierre MARTINEZ

Philippe MD PICQUENDAR

François MD BALAYRE

Corinne MD DEBEAUVAIS

Marion MD FINET

Olivier MD JAEGGY

Doriane MD CHACUNE

Valérie MD SZONYI

Maud MD GRAMMATICA

Nancie MD COUGOT

Loïc MD CALVO

Jérémy MD GLOMET

François-Marie MD DUTOUR

Jad MD MASRI

Xavier MD BONDIL

Nathalie MD JANISSET-MASSE

Head and neck cancers represent more than 500,000 cases per year worldwide, and often involve post-treatment relapse. The oral cavity is the most frequent site, but early disease stages are still insufficiently characterised and poorly detected. The study's aim is to better understand the oral mucosa somatic evolution, and how it can give rise to oral squamous cell carcinoma (OSCC).

This is a multi-centric, descriptive, non-interventional cohort in healthy adult subjects. The aim of the study is to detect the presence of OSCC driver gene mutations in healthy subjects' oral mucosa, quantify whether they provide cells with a selective advantage, and study the impact of tobacco and alcohol consumption on the mutational load. This study will not allow to identify potential malignant cells, and will be usable for diagnostic purposes.

Oral cytobrush samples will be collected (1 per patient, single time point without follow-up) and analysed via deep whole exome sequencing (\>500x).

Tobacco and Alcohol Influence on Oncogenic Drivers and Somatic Evolution in the Oral Mucosa

Difference in mean number of non-synonymous mutations found in OSCC driver genes between smokers and non-smokers.

Hospices Civils de Lyon

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
Tobacco and Alcohol Influence on Oncogenic Drivers and Somatic Evolution in the Oral Mucosa	NCT06094829	Carcinoma, Squa...	Oral cytobrush	18 Years -	Hospices Civils de Lyon
Tobacco and Alcohol Influence on Oncogenic Drivers and Somatic Evolution in the Oral Mucosa	NCT06094829	Carcinoma, Squa...	Oral cytobrush	18 Years -	Hospices Civils de Lyon
Tobacco and Alcohol Influence on Oncogenic Drivers and Somatic Evolution in the Oral Mucosa	NCT06094829	Carcinoma, Squa...	Oral cytobrush	18 Years -	Hospices Civils de Lyon

Spots Global Cancer Trial Database for tobacco and alcohol