Spots Global Cancer Trial Database for FOS Immunohistochemical Staining of Colorectal Cancer and Its Adjacent Tissues
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Trial Identification
Brief Title: FOS Immunohistochemical Staining of Colorectal Cancer and Its Adjacent Tissues
Official Title: Monkeypox and Cancer: a Pan-cancer Based Multi-omics Analysis and Single Cell Sequencing Analysis-Experimental Studies
Study ID: NCT05698082
Interventions
Study Description
Brief Summary: Colorectal cancer tissue sections were obtained according to the inclusion criteria. The formalin was used to immersed all cancer specimens. And tissues were cut to 5 μm thickness and placed on glass slides before staining. Endogenous peroxidase activity was inhibited and blocked by de-paraffinizing, rehydrating, and using 5% bovine serum albumin at 37ºC for 30 min. The treated sections were incubated with anti-FOS (promab 30360) at 4ºC overnight and washed three times with PBS. After that, it is required that incubation with secondary anti-peroxidation sunflower at 37ºC for 30 minutes. After washing three times again with PBS, the sections were developed in diaminobenzidine and microscopic images were made by light microscopy.
Detailed Description:
Eligibility
Minimum Age: 18 Years
Eligible Ages: ADULT, OLDER_ADULT
Sex: MALE
Healthy Volunteers: No
Locations
Guangxi Medical University Cancer Hospital, Nanning, Guangxi, China
Contact Details
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