Neoplasms

Eye Diseases

All Conditions

Diseases and Abnormalities at or Before Birth

Rare Diseases

Retinoblastoma

Neoplasms, Neuroepithelial

Neuroectodermal Tumors

Neuroectodermal Tumors, Primitive

Neoplasms, Germ Cell and Embryonal

Neoplasms by Histologic Type

Neoplasms, Glandular and Epithelial

Neoplasms, Nerve Tissue

Retinal Neoplasms

Eye Neoplasms

Eye Diseases, Hereditary

Retinal Diseases

Neuroepithelioma

Tissue samples taken from retinoblastoma

White blood cells will be isolated from the blood sample as a source of genomic DNA.

5 ml peripheral blood

Rachel Brennan, MD

Retinoblastoma is a childhood tumor of the retina that affects approximately 1 in 18,000 children. Retinoblastoma is more prevalent in Central America, where most patients present with advanced intraocular and extraocular disease. Therefore, we have formed collaboration with Central American investigators to provide us with frozen retinoblastoma tumors from patients undergoing routine enucleation. The objective of this protocol is to begin to identify the mutations and gene expression changes that occur in retinoblastoma cells following RB1 gene inactivation in order to more effectively target chemotherapy for treating bilateral retinoblastoma.

This is a biology protocol with no treatment regimen associated. Following routine enucleation of the retinoblastoma affected eye, a portion of the tumor will be removed and flash frozen and sent to St. Jude for RNA and DNA analysis. Some tissue will undergo histopathological analysis and tissue microarrays will be isolated.

* Each tumor sample will be immediately transferred to a sterile cryovial and flash frozen in liquid nitrogen.
* White blood cells will be isolated from the blood sample as a source of genomic DNA.

Molecular Analysis of Retinoblastoma

National Eye Institute (NEI)

St. Jude Children's Research Hospital

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
Molecular Analysis of Retinoblastoma	NCT00718783	Retinoblastoma	Tissue samples ... 5 ml peripheral...	-	St. Jude Children's Research Hospital
Molecular Analysis of Retinoblastoma	NCT00718783	Retinoblastoma	Tissue samples ... 5 ml peripheral...	-	St. Jude Children's Research Hospital

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