Neoplasms

All Conditions

Mouth and Tooth Diseases

Rare Diseases

Carcinoma

Salivary Gland Neoplasms

Mouth Neoplasms

Head and Neck Neoplasms

Mouth Diseases

Stomatognathic Diseases

Salivary Gland Diseases

Oral Cancer

Initial diagnosis of primary salivary gland carcinoma in the head and neck region

Initial diagnosis of a benign salivary gland tumor in the head and neck region

Healthy control group. Functional diseases of the nose or ear (patients with the indication for functional ear surgery and rhinoplasty) without salivary gland tumor.

Evaluation of immune characteristics by using patient's stool, saliva and blood samples.

Sampling

Studiensekretariat

Translation Radiobiology

Sarina Mueller, PD

Marlen Haderlein, PD

Benjamin Frey, PD

Aims of this study are analyses of tumor metabolome, tumor transcriptome and tumor proteome as well as of the immune infiltration, separated by histological entity. These data will subsequently be compared with the with the detailed immune status determined in the patient's peripheral blood and saliva using machine learning techniques, among others, to create a biomarker cluster for salivary gland tumors. These can be used in clinical routine.

In addition, the investigators would like to study a subset of patients from freshly resected tumor organoids from freshly resected tumor tissue according to already established methods in order to mechanistic investigations of prognostic parameters.

In this study, the prognostic value of local immunological tumor-dependent cell populations will be prospectively investigated and correlated with immunological changes in the blood. Not only single immune cell populations but also the immune score of multiple immune cells or immune cell subsets will be investigated. In addition, the systemic immune status of patients in peripheral blood will be studied longitudinally. Furthermore, the analysis of cytokines and chemokines as well as mRNA and ctDNA in the peripheral blood is blood and the determination of transcription processes in immune cells, especially to extend the especially to extend the prognostic immune signature.

Immune Biomarker Study for Salivary Gland Carcinoma

Different immune cells and tumor cell markers will characterize immunological groups using cluster analysis. Immune matrix of patients assessed by liquid immune profile-based signature (LIPS) (acc. Zhou et al. Journal for ImmunoTherapy of Cancer (JITC), 2021) and Tumour Associated Lymphocytes (TAL).

The distribution of immune cells and messenger substances in the blood will be examined by means of immunophenotyping in order to add the systemic immune cell composition.

Flow cytometric assessment of the amount of circulating immune cell-distribution per milliliter whole blood according to the LIPS technique (Zhou et al. JITC 2021).

Electrochemiluminescent MULTI-ARRAY measurement of concentration (pg/ml whole blood) cytokines/chemoattractant cytokines in the serum/plasma of the patients according to the LIPS technique (Zhou et al. JITC 2021).

Mass-spectrometric untargeted metabolomic of patients serum/plasma to assess the change of metabolites (pg/ml whole blood) from baseline to end of radiotherapy.

16S rRNA deep sequencing of microbiome in salvia, tumour and stool samples to assess the presence and relative distribution of microbiotes (Operational taxonomic units (OTUs)).

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
Immune Biomarker Study for Salivary Gland Carcinoma	NCT06047236	Salivary Gland ... Benign Salivary...	Sampling	18 Years -	University of Erlangen-Nürnberg Medical School
Immune Biomarker Study for Salivary Gland Carcinoma	NCT06047236	Salivary Gland ... Benign Salivary...	Sampling	18 Years -	University of Erlangen-Nürnberg Medical School

Spots Global Cancer Trial Database for benign salivary gland tumor