Neoplasms

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Gastrointestinal Neoplasms

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Use of Saccharomyces boulardii, 100mg for at least seven days before surgery

100mg daily for seven days prior to surgery

Saccharomyces boulardii

Maria Isabel Correia, MD, PhD

The purpose of this study is to assess the impact of probiotic administration (Saccharomyces boulardii) on patients undergoing colorectal resections comparing to the routine protocol by assessing: (1)intestinal microbiota modulation using RT-PCR to measure cytokine in the mucosa; (2) assess postoperative complications, mainly infectious and mortality, as well as length of hospital stay

The intestinal microbiota, a complex and dynamic population of different bacterial species, under normal circumstances, represents an important contribution to the health of the host. This plays a key role by maintaining the integrity of the epithelial barrier and helping the development of mucosal immunity. However, under some stressful situations, such as after gastrointestinal surgery, infectious complications may be originated from the patient's own intestinal microbiota. This leads to the so called "gut origin of sepsis" hypothesis. On the other hand, under similar conditions, the supply of probiotics, the good bacteria, has been shown to be beneficial, despite few controversial results. Therefore, it is important to carefully assess the efficacy of probiotics in the prevention and treatment of complications in surgical patients, as well as to evaluate the safety of its use.

Impact of Probiotics on the Intestinal Microbiota

Impact of Probiotics on the Intestinal Microbiota and Its Association With Postoperative Outcome After Colorectal Surgery

Federal University of Minas Gerais

Professor of Surgery, responsible investigator

Infections

Symptoms and General Pathology

Communicable Diseases

Herbal and Botanical

All Drugs and Chemicals

Cola

Conventional treatment with no probiotic supplementation

The patients received one oral lyophilized yeast capsule, each of which contains 100 mg (0,5 x 109 cfu/g) of Saccharomyces boulardii (Merck S.A., Biocodex, Beauvais, French), once a day. The treatment started at least seven days before surgery and stopped on the operation day.

Isabel TD Correia, PhD

The investigators would study about impact of the administration of probiotics in the intestinal mucosa of patients undergoing resection colic, by evaluating cytokine profile by quantitative real time PCR. The investigators believe that patients who use probiotic preoperative would provide cytokine profile less inflammatory than those of the control group.

Samples of colorectal cancer-free mucosa derived from patients who underwent surgical resection were investigated. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to determine expression levels of the following genes: IL10, IL1B, IL23A, TNF, IL12B, INFG, IL17A.

Impact of Probiotics in Modulation of Intestinal Microbiota

Impact of Probiotics in Modulation of Intestinal Microbiota of Patients Undergoing a Resection Colonic

Colonic mucosal specimens were analyzed to determine the mucosal expression proﬁles of IL1B, IL12B, IL10, IL23A, INFG, IL17A and TNF.

Postoperative complications assessed up to 30 days

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
Impact of Probiotics on the Intestinal Microbiota	NCT01609660	Colorectal Canc...	Saccharomyces b...	18 Years -	Federal University of Minas Gerais
Impact of Probiotics in Modulation of Intestinal Microbiota	NCT01895530	Colorectal Canc...	Saccharomyces b...	-	Federal University of Minas Gerais
Impact of Probiotics in Modulation of Intestinal Microbiota	NCT01895530	Colorectal Canc...	Saccharomyces b...	-	Federal University of Minas Gerais

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