Infections

All Conditions

Neoplasms

Urinary Tract, Sexual Organs, and Pregnancy Conditions

Symptoms and General Pathology

Uterine Cervical Neoplasms

Communicable Diseases

Papillomavirus Infections

Disease Attributes

Sexually Transmitted Diseases

Sexually Transmitted Diseases, Viral

Virus Diseases

DNA Virus Infections

Tumor Virus Infections

Genital Diseases

Urogenital Diseases

Uterine Neoplasms

Genital Neoplasms, Female

Urogenital Neoplasms

Uterine Cervical Diseases

Uterine Diseases

Genital Diseases, Female

Female Urogenital Diseases

Pregnancy Complications

Female Urogenital Diseases and Pregnancy Complications

volunteers(n = 50/center) doing gynecologial examination recruited from health examination center, who shows no symptoms and signs in reproductive tract and are potentially regarded as the healthy controls

volunteers(n = 150/center) recruited from gynecology outpatient, who show abnormal symptoms and signs in reproductive tract and are potentially regarded as the cases with conditions in reproductive tract

1. 16S rRNA gene sequencing of vaginal secretions: using NGS technology to assess the microbiota of the vaginal secretions
2. Microecology assessment of vaginal secretions: morphology test, functional assessment and Nugent score assessment of the vaginal secretions are included.
3. Genotyping of HPV and detection of sexually transmitted diseases pathogens based on the cervical exfoliated cells
4. Thinprep cytologic test of cervical exfoliated cells: to evaluate the grade of cervical intraepithelial neoplasia

16S rRNA gene sequencing of cervical secretions

Muxuan Chen

Ming Li

Haitao Yu

Zewei Huang

Bashan Zhang

Chaoxin Jiang

Mu-Xuan Chen

Lei Zheng

Qunxiang Liu

Taoyu Zhou

Kewei Zhao

Xuefeng Wang

Xianjin Wu

Ling Ji

Bohai Yu

Xiuming Zhang

Zhaofan Luo

Jiuxin Qu

Guanghui Chen

Shuzhong Yao

Gongjun Tan

Fan Lu

Kaifeng Wu

Pingsen Zhao

Bo Wang

Xianghua Huang

Hong Chen

Wei Liang

Guijie Zhang

Feng Li

Dengyun Ma

Shuyi Han

Chunmei Ying

Yan Han

Ruifang An

Jinbo Liu

Zhi Juan

Xumei Zhang

Jun Zhang

Xuelian Zhang

Kun Deng

Liang Peng

Jingxin Cheng

Hongwei Zhou, Doctor

The purpose of this study to access the relations between genital microecology, HPV infection and cervical intraepithelial neoplasia of childbearing-age female in China

The volunteers will be recruited from the women who come to the hospital to do the thinprep cytologic test. After being informed about the potential risks, each volunteer giving written informed consent will be asked to complete a questionnaire. Then, cervical and vaginal swabs and cervical exfoliated cells will be taken from each volunteer to perform 16S rRNA gene sequencing microecology assessment on cervical secretions and detection and genotyping of HPV and pathogens of sexually transmitted diseases. If there is something abnormal with the diagnostic outcomes in the previous examinations, according to the doctor's judgement, those volunteer will be asked to undergo a vaginoscope and a cervical biopsy to evaluate the grade of cervical intraepithelial neoplasia. After six months since the entry, each volunteer will be asked to go back to each research center to do the same sampling and examination as previously did in entry.

Biospecimen will include cervical and vaginal swabs, cervical exfoliated cells from reproductive tract of the volunteers

HPV Infection and Genital Microecology of Childbearing-age Female in China: A Cohort and Multicenter Study

Genital microecology includes measurements of cervical microbiota, HPV and other sexually transmitted diseases pathogens infection status, conditions of the vaginal secretion and the grade of cervical intraepithelial neoplasia. As for cervical microbiota, the NGS technology will be used to sequence the 16s rRNA gene of the microbes in the cervical secretion of each volunteers. PCR will be used to detect HPV and other sexually transmitted diseases pathogens. As for the conditions of the vaginal secretion, methods such as manual microscopic inspection, gram stain and enzymology test will be used to do the measurement. Moreover, thinprep cytologic test of cervical exfoliated cells will be performed to evaluate the grade of cervical intraepithelial neoplasia.

Peking University Hospital

The Affiliated Hospital Of Guizhou Medical University

Hainan People's Hospital

LanZhou University

Lianyungang Hospital Affiliated Bengbu Medical College

General Hospital of Ningxia Medical University

Jinan Central Hospital

First Affiliated Hospital Xi'an Jiaotong University

Tibet Autonomous Region People's Hospital

The Affiliated Hospital Of Southwest Medical University

Maternal and Child Health Hospital of Xinjiang Uygur Autonomous Region

Yan'an Hospital of Kunming City

The First Affiliated Hospital of University of Science and Technology of China

Zunyi First People's Hospital

Peking University Shenzhen Hospital

Dongguan People's Hospital

Guangdong Provincial Hospital of Integrated Traditional Chinese and Western Medicine

The Third Affiliated Hospital of Southern Medical University

Taihe Branch of Nanfang Hospital

First Affiliated Hospital, Sun Yat-Sen University

Shenzhen Luohu Hospital Group Luohu People's Hospital

Zhuhai Center for Maternal and Child Health Care

Obstetrics & Gynecology Hospital of Fudan University

First Affiliated Hospital of Guangxi Medical University

The Second Hospital of Hebei Medical University

Jilin Central General Hospital

The First Hospital fo Qiqihar

Qinghai Red Cross Hospital

Changzhi People's Hospital

Shanghai East Hospital Tongji University

Sir Run Run Shaw Hospital

The Third Affiliated Hospital of Guangzhou University of Chinese Medicine

Fifth Affiliated Hospital of Guangzhou Medical University

Shenzhen Hospital of Guangzhou University of Chinese Medicine

Huizhou Municipal Central Hospital

First Affiliated Hospital of Jinan University

Nanfang Hospital, Southern Medical University

Shenzhen Third People's Hospital

Yuebei People's Hospital

The Seventh Affiliated Hospital of Sun Yat-sen University

Xiaolan People's Hospital of Zhongshan

Chaozhou Central Hospital

The Third Affiliated Hospital of CQMU

Changsha Central Hospital

Zhujiang Hospital

Diagnostic Technician

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization	Link
HPV Infection and Genital Microecology of Childbearing-age Female in China: A Cohort and Multicenter Study	NCT04694495	Papillomavirus ... Uterine Cervica... Sexual Transmit...	16S rRNA gene s...	18 Years - 50 Years	Zhujiang Hospital
HPV Infection and Genital Microecology of Childbearing-age Female in China: A Cohort and Multicenter Study	NCT04694495	Papillomavirus ... Uterine Cervica... Sexual Transmit...	16S rRNA gene s...	18 Years - 50 Years	Zhujiang Hospital

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