Neoplasms

All Conditions

Rare Diseases

Neuroendocrine Tumors

Neuroectodermal Tumors

Neuroectodermal Tumors, Primitive

Neoplasms, Germ Cell and Embryonal

Neoplasms by Histologic Type

Neoplasms, Nerve Tissue

Neuroendocrine Tumor

Neuroepithelioma

Collect biopsy/surgical fresh tissue of gastroenteropancreatic neuroendocrine neoplasms.

Collect biopsy/surgical fresh tissue of pancreatic ductal adenocarcinoma.

Collect NEN and PDAC biopsy or surgical fresh tissue to conduct single cell sequencing or model construction.

Biopsy, open or laparoscopic surgery

Xianjun Yu, MD, PhD

Xianjun Yu, M.D., Ph.D.

The aim of this study is to use single-cell sequencing technology to explore neuroendocrine neoplasm (NEN) molecular biological characteristics, tumor heterogeneity and cell subtypes. Besides. NEN models are constructed for basic research, including primary cell lines, organoids, and animal models.

Neuroendocrine neoplasm (NEN) generally refers to all tumors that originate from peptidergic neurons and neuroendocrine cells. It is a relatively rare tumor, ranging from indolent, slow-growing low-grade malignant tumors to obvious distant metastases. A series of malignant tumors. The most common place for neuroendocrine tumors is in the digestive system, and about two-thirds of neuroendocrine tumors occur in the gastrointestinal pancreas. However, with the change of people's living habits and the improvement of physical examination awareness, the incidence of neuroendocrine tumors increased from 1.09/100,000 in 1973 to 6.98/100,000 in 2012. The incidence has increased by 6 times compared with other tumors. , NENs increase more rapidly. The incidence of people over 65 years of age has increased by 8 times, and the incidence of people younger than 50 years of age has also increased by 3 times, and the incidence has been increasing year by year. It is worth noting that the average age of pancreatic neuroendocrine tumors is only 56.7-13.3 years old, showing a younger trend.

Because most neuroendocrine tumors lack specific manifestations, coupled with unique inert biological characteristics, clinicians often lack understanding, which can easily lead to misdiagnosis and treatment. Taking gastrointestinal pancreatic neuroendocrine tumors (GEP-NEN) as an example, due to the uneven diagnosis and treatment level, GEP-NEN patients may be diagnosed with a delay of up to 7 years. Because of this, as many as 40-95% of GEP-NEN patients have developed distant metastases at the time of diagnosis, and 65-95% of them have liver metastases. Liver metastasis is the most critical prognostic risk factor in GEP-NEN, and it has a decisive impact on the survival of patients. The 5-year survival rate of gastrointestinal neuroendocrine tumors with liver metastasis is 56%-83%, while the 5-year survival rate of pancreatic neuroendocrine tumors is only 48.8%, and the 10-year survival rate is only 30.2%. For limited-stage patients, even if radical surgical resection, up to 94% of cases will still have recurrence and metastasis within 5 years. For inoperable patients, although chemotherapy, targeted therapy, biological therapy and other methods can be used, the effect is still limited. Therefore, the systematic development of basic-clinical translational research on neuroendocrine tumors is very important and imminent.

Based on the above-mentioned problems, this project intends to conduct a systematic and in-depth study of neuroendocrine tumors: 1. Use single-cell sequencing technology to deeply study the molecular biological characteristics of NEN tumors, tumor heterogeneity and cell subtypes. 2. Construct NEN models, including primary cell lines, organoids, and mouse animal models. This study can establish a neuroendocrine tumor research system to find the molecular mechanism and potential intervention targets of NEN recurrence and metastasis, and provide clinicians with safe and effective treatment strategies, thereby improving the therapeutic effect of neuroendocrine tumors.

Single-cell Sequencing and Establishment of Models in Neuroendocrine Neoplasm

Single-cell Sequencing and Establishment of Cell Lines, Organoids, and Transplanted Tumor Models in Neuroendocrine Neoplasm

Analysis of NEN Molecular Biology Information Using Single Cell Sequencing Technology

Collect fresh specimens of NEN to cultivate primary cell lines, construct organoids, and establish animal models of NEN

To explore and optimize the medium scheme of NEN orgnoid and methods of histomorphological identification of type organs

The tumor microenvironment of NEN was analyzed

Collect fresh specimens of pancreatic ductal adenocarcinoma (PDAC) for single-cell sequencing and organoid construction for comparison with NEN

D1 Medical Technology (Shanghai) Co., Ltd, China

Fudan University

President of Shanghai Pancreatic Cancer Institute

Respiratory Tract (Lung and Bronchial) Diseases

Symptoms and General Pathology

Musculoskeletal Diseases

Blood and Lymph Conditions

Lung Neoplasms

Neoplasm Metastasis

Neoplastic Cells, Circulating

Bone Neoplasms

Bone Marrow Diseases

Neoplastic Processes

Bone Diseases

Hematologic Diseases

the treatment decisions were decided by Machine Learning （ML） model in model treatment group

model treatment

the treatment decisions were decided by multi-disciplinary team （MDT）

routine treatment

hui zhao

1. Evaluate the feasibility of single cell sequencing technology based on three-dimensional bionic capture network;
2. To build a risk prediction model of bone related events based on single cell sequencing;
3. To verify the risk prediction model of bone related events by single cell sequencing;
4. To verify the comprehensive treatment of non-small cell lung cancer ( NSCLC) bone metastases.

CTC in Lung Caner Patients With Bone Metastases

Clinical Reasearch on Three Dimensional Bionic Capture Network Circulating Tumor Cell in Lung Caner Patients With Bone Metastases

occurance of SREs，including bone pain, pathological fractures, spinal cord compression, hypercalcemia, and the need for surgery or radiotherapy

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
Single-cell Sequencing and Establishment of Models in Neuroendocrine Neoplasm	NCT04927611	Neuroendocrine ...	Biopsy, open or...	18 Years - 80 Years	Fudan University
CTC in Lung Caner Patients With Bone Metastases	NCT04568291	Lung Cancer Bone Metastases Single Cell Seq...	model treatment routine treatme...	18 Years - 75 Years	Shanghai 6th People's Hospital
Single-cell Sequencing and Establishment of Models in Neuroendocrine Neoplasm	NCT04927611	Neuroendocrine ...	Biopsy, open or...	18 Years - 80 Years	Fudan University

Spots Global Cancer Trial Database for single cell sequencing technology