Neoplasms

Urinary Tract, Sexual Organs, and Pregnancy Conditions

Diseases and Abnormalities at or Before Birth

All Conditions

Symptoms and General Pathology

Rare Diseases

Wilms Tumor

Kidney Neoplasms

Neoplasms by Histologic Type

Urologic Neoplasms

Urogenital Neoplasms

Syndrome

Neoplastic Syndromes, Hereditary

Urogenital Diseases

Female Urogenital Diseases

Pregnancy Complications

Female Urogenital Diseases and Pregnancy Complications

Kidney Diseases

Urologic Diseases

Male Urogenital Diseases

Genetic Diseases, Inborn

Wilms' Tumor

Paraffin-embedded specimens are analyzed for macrophage markers. Results of each sample are then compared with patient's tumor stage, presence of vascular invasion, tumor progression, and survival.

laboratory biomarker analysis

Jessica Kandel, MD

This research study is studying biomarkers in tissue samples from younger patients with Wilms tumor. Studying samples of tissue from patients with cancer in the laboratory may help doctors identify and learn more about biomarkers related to cancer. It may also help doctors predict how patients respond to treatment

PRIMARY OBJECTIVES:

I. To determine whether recruitment of tumor-associated macrophages is related to clinicopathological factors in Wilms tumor (tumor stage, presence of vascular invasion, tumor progression, and survival).

OUTLINE:

Paraffin-embedded specimens are analyzed for macrophage markers. Results of each sample are then compared with patient's tumor stage, presence of vascular invasion, tumor progression, and survival.

Biomarkers in Samples From Younger Patients With Wilms Tumor

Observational - Relationships Between Tumor-Associated Macrophages and Clinicopathological Factors in Wilms Tumor

Correlation of each type of macrophage score with tumor staging, presence of vascular invasion, and disease-free survival will be assessed.

National Cancer Institute (NCI)

Children's Oncology Group

Carcinoma, Renal Cell

Renal Cell Carcinoma

DNA analysis

nucleic acid amplification

diagnostic laboratory biomarker analysis

medical chart review

Elizabeth J. Perlman, MD

RATIONALE: Studying samples of blood, tissue, or bone marrow from patients with cancer in the laboratory may help doctors to learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research trial studies biomarkers in samples from younger patients with Wilms tumor.

OBJECTIVES:

* To analyze 250 favorable-histology Wilms tumor (FHWT) samples from patients registered on NWTS4 for gain of 1q and 15q, and loss of 1p, 16q, 17p, and 22q by subtelomeric multiplex ligation-dependent probe amplification (MLPA), and to determine their association with relapse-free and overall survival.
* To develop a robust multiplex MLPA test using multiple synthesized probes to those targets identified as significantly associated with relapse in Aim 1.
* To validate the multiplex MLPA test designed and tested in Aim 2 on an independent set of 1727 FHWT and 250 anaplastic WT registered on NWTS-5.

OUTLINE: Archived DNA samples are analyzed for gain of 1q and 15q, and loss of 1p, 16q, 17p, and 22q by MLPA. Assay results are then researched for accuracy, clinical validity, sensitivity, precision, and potential analytical interferences.

Studying Biomarkers in Samples From Younger Patients With Wilms Tumor

Validation of Copy Number Changes by MLPA as Predictors of Relapse in Wilms Tumor

DNA methylation analysis

RNA analysis

allele-specific oligonucleotide real-time quantitative polymerase chain reaction

gene expression analysis

nucleic acid sequencing

polymorphism analysis

Karin Michels, MD, PhD

RATIONALE: Studying samples of blood and tissue from newborns and from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research trial studies cord blood and placenta tissue from newborns, and tumor tissue samples from patients with Wilms tumor.

OBJECTIVES:

* What is the frequency of loss of imprinting at birth (in the cord blood and placenta) in a relatively healthy birth cohort?
* Does deoxyribonucleic acid (DNA) methylation levels at imprinting genes have a direct association to the gene expression?

OUTLINE: Archived tumor tissue, cord blood, and placenta samples are analyzed for DNA methylation, single nucleotide polymorphism, and gene expression by polymerase chain reaction (PCR), pyrosequencing, and quantitative real-time PCR. Information regarding gender and age of the samples are also collected, if possible.

Studying Genes Using Cord Blood and Placenta Samples From Relatively Healthy Newborns and Samples From Younger Patients With Wilms Tumor

Investigating the Frequency of Loss of Imprinting Across a Birth Cohort and the Link DNA Methylation Plays

Study title	NCT ID	Conditions	Interventions	Eligibility	Organization
Biomarkers in Samples From Younger Patients With Wilms Tumor	NCT01493817	Wilms Tumor and...	laboratory biom...	-	Children's Oncology Group
Studying Biomarkers in Samples From Younger Patients With Wilms Tumor	NCT01576198	Kidney Cancer	DNA analysis nucleic acid am... diagnostic labo... medical chart r...	- 120 Years	Children's Oncology Group
Studying Genes Using Cord Blood and Placenta Samples From Relatively Healthy Newborns and Samples From Younger Patients With Wilms Tumor	NCT01576211	Kidney Cancer	DNA methylation... RNA analysis allele-specific... gene expression... nucleic acid se... polymorphism an... laboratory biom...	- 21 Years	Children's Oncology Group
Studying Biomarkers in Samples From Younger Patients With Wilms Tumor	NCT01576198	Kidney Cancer	DNA analysis nucleic acid am... diagnostic labo... medical chart r...	- 120 Years	Children's Oncology Group

Spots Global Cancer Trial Database for wilms tumor and other childhood kidney tumors